The proximate analysis and antimicrobial activities of Persea Americana seed (Avocado pear) against pathogenic Escherichia coli and Staphylococcus aureus was carried out to ascertain the unique properties that makes it confer antibacterial effect. This research work was carried out in the Microbiology Project Laboratory of Chukwuemeka Odumegwu Ojukwu University, Uli. The organisms used were obtained from urine samples of students and characterized using microbiological and biochemical tests. The phytochemical and proximate analysis of the seed extract of the plant was determined quantitatively using chemical and spectrophotometric methods. The inhibitory activity test was carried out using agar well diffusion method also tube dilution technique using double-fold serial dilution method was employed for assaying the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) at various concentrations (31.25, 62.5, 125, 250 and 500 mg/ml). The phytochemical analysis revealed the presence of alkaloids, tannins, flavonoids, saponins, phenols, cyanogenic glycosides and steroids. The proximate content revealed that the seed contained more of moisture than fat and fibre. The ethanolic extract of Persea americana seed showed a pronounced activity (11.00 mm and 9.40 mm) against Escherichia coli and Staphylococcus aureus were significantly higher (P<0.05) than that of the aqueous extract (5.90 mm) which showed for only Escherichia coli. This activity differed significantly (P>0.05) from that of Ciprofloxacin (22.10 mm and 16.40 mm). The results of MIC for the ethanolic extract against E. coli and S. aureus were 500 mg/ml and 250 mg/ml respectively, while the MBC result was pronounced for only E. coli at 500 mg/ml. The spectra of antimicrobial activities displayed by the extracts could be attributed to the presence of these phytochemicals and signifies the potential of Persea americana as a source of therapeutic agent.
| Published in | Journal of Diseases and Medicinal Plants (Volume 4, Issue 3) |
| DOI | 10.11648/j.jdmp.20180403.14 |
| Page(s) | 89-95 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2018. Published by Science Publishing Group |
Escherichia coli, Staphylococcus aureus, Persea Americana Seed, MIC, MBC
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APA Style
Umeaku Chinyelu Nkiru, Chris-Umeaku Chiamaka Ijeoma, Emmy-egbe Ifeyinwa Orsla, Ukoha Chinwe Clarice, Uzor Ugonwa Chiagoziem, et al. (2018). Proximate, Phytochemical and Antibacterial Analysis of Persea americana Obtained from Nigeria. Journal of Diseases and Medicinal Plants, 4(3), 89-95. https://doi.org/10.11648/j.jdmp.20180403.14
ACS Style
Umeaku Chinyelu Nkiru; Chris-Umeaku Chiamaka Ijeoma; Emmy-egbe Ifeyinwa Orsla; Ukoha Chinwe Clarice; Uzor Ugonwa Chiagoziem, et al. Proximate, Phytochemical and Antibacterial Analysis of Persea americana Obtained from Nigeria. J. Dis. Med. Plants 2018, 4(3), 89-95. doi: 10.11648/j.jdmp.20180403.14
AMA Style
Umeaku Chinyelu Nkiru, Chris-Umeaku Chiamaka Ijeoma, Emmy-egbe Ifeyinwa Orsla, Ukoha Chinwe Clarice, Uzor Ugonwa Chiagoziem, et al. Proximate, Phytochemical and Antibacterial Analysis of Persea americana Obtained from Nigeria. J Dis Med Plants. 2018;4(3):89-95. doi: 10.11648/j.jdmp.20180403.14
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Download@article{10.11648/j.jdmp.20180403.14,
author = {Umeaku Chinyelu Nkiru and Chris-Umeaku Chiamaka Ijeoma and Emmy-egbe Ifeyinwa Orsla and Ukoha Chinwe Clarice and Uzor Ugonwa Chiagoziem and Agbo Uchenna Juliet},
title = {Proximate, Phytochemical and Antibacterial Analysis of Persea americana Obtained from Nigeria},
journal = {Journal of Diseases and Medicinal Plants},
volume = {4},
number = {3},
pages = {89-95},
doi = {10.11648/j.jdmp.20180403.14},
url = {https://doi.org/10.11648/j.jdmp.20180403.14},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jdmp.20180403.14},
abstract = {The proximate analysis and antimicrobial activities of Persea Americana seed (Avocado pear) against pathogenic Escherichia coli and Staphylococcus aureus was carried out to ascertain the unique properties that makes it confer antibacterial effect. This research work was carried out in the Microbiology Project Laboratory of Chukwuemeka Odumegwu Ojukwu University, Uli. The organisms used were obtained from urine samples of students and characterized using microbiological and biochemical tests. The phytochemical and proximate analysis of the seed extract of the plant was determined quantitatively using chemical and spectrophotometric methods. The inhibitory activity test was carried out using agar well diffusion method also tube dilution technique using double-fold serial dilution method was employed for assaying the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) at various concentrations (31.25, 62.5, 125, 250 and 500 mg/ml). The phytochemical analysis revealed the presence of alkaloids, tannins, flavonoids, saponins, phenols, cyanogenic glycosides and steroids. The proximate content revealed that the seed contained more of moisture than fat and fibre. The ethanolic extract of Persea americana seed showed a pronounced activity (11.00 mm and 9.40 mm) against Escherichia coli and Staphylococcus aureus were significantly higher (PEscherichia coli. This activity differed significantly (P>0.05) from that of Ciprofloxacin (22.10 mm and 16.40 mm). The results of MIC for the ethanolic extract against E. coli and S. aureus were 500 mg/ml and 250 mg/ml respectively, while the MBC result was pronounced for only E. coli at 500 mg/ml. The spectra of antimicrobial activities displayed by the extracts could be attributed to the presence of these phytochemicals and signifies the potential of Persea americana as a source of therapeutic agent.},
year = {2018}
}
TY - JOUR T1 - Proximate, Phytochemical and Antibacterial Analysis of Persea americana Obtained from Nigeria AU - Umeaku Chinyelu Nkiru AU - Chris-Umeaku Chiamaka Ijeoma AU - Emmy-egbe Ifeyinwa Orsla AU - Ukoha Chinwe Clarice AU - Uzor Ugonwa Chiagoziem AU - Agbo Uchenna Juliet Y1 - 2018/09/19 PY - 2018 N1 - https://doi.org/10.11648/j.jdmp.20180403.14 DO - 10.11648/j.jdmp.20180403.14 T2 - Journal of Diseases and Medicinal Plants JF - Journal of Diseases and Medicinal Plants JO - Journal of Diseases and Medicinal Plants SP - 89 EP - 95 PB - Science Publishing Group SN - 2469-8210 UR - https://doi.org/10.11648/j.jdmp.20180403.14 AB - The proximate analysis and antimicrobial activities of Persea Americana seed (Avocado pear) against pathogenic Escherichia coli and Staphylococcus aureus was carried out to ascertain the unique properties that makes it confer antibacterial effect. This research work was carried out in the Microbiology Project Laboratory of Chukwuemeka Odumegwu Ojukwu University, Uli. The organisms used were obtained from urine samples of students and characterized using microbiological and biochemical tests. The phytochemical and proximate analysis of the seed extract of the plant was determined quantitatively using chemical and spectrophotometric methods. The inhibitory activity test was carried out using agar well diffusion method also tube dilution technique using double-fold serial dilution method was employed for assaying the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) at various concentrations (31.25, 62.5, 125, 250 and 500 mg/ml). The phytochemical analysis revealed the presence of alkaloids, tannins, flavonoids, saponins, phenols, cyanogenic glycosides and steroids. The proximate content revealed that the seed contained more of moisture than fat and fibre. The ethanolic extract of Persea americana seed showed a pronounced activity (11.00 mm and 9.40 mm) against Escherichia coli and Staphylococcus aureus were significantly higher (PEscherichia coli. This activity differed significantly (P>0.05) from that of Ciprofloxacin (22.10 mm and 16.40 mm). The results of MIC for the ethanolic extract against E. coli and S. aureus were 500 mg/ml and 250 mg/ml respectively, while the MBC result was pronounced for only E. coli at 500 mg/ml. The spectra of antimicrobial activities displayed by the extracts could be attributed to the presence of these phytochemicals and signifies the potential of Persea americana as a source of therapeutic agent. VL - 4 IS - 3 ER -
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