Growth and Accumulation of Five Main Bioactive Components in the Roots of <i>Salvia miltiorrhiza</i> at Different Growth Stages and Using Different Culture Systems

Lilan Lu; Shilong Fu; Jian Feng; Jianhe Wei

doi:doi:10.11648/j.jdmp.20180403.11

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Growth and Accumulation of Five Main Bioactive Components in the Roots of Salvia miltiorrhiza at Different Growth Stages and Using Different Culture Systems

Lilan Lu, Shilong Fu, Jian Feng, Jianhe Wei

Published in Journal of Diseases and Medicinal Plants (Volume 4, Issue 3)

Received: 23 April 2018 Accepted: 21 May 2018 Published: 8 June 2018

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Abstract

To Growth and accumulation of five main bioactive components in the roots of Salvia miltiorrhiza at different growth stages and using different culture systems. We analyzed growth parameters and the accumulation of selected bioactive components in Salvia miltiorrhiza that was grown in quartz sand-pot (hydroponic culture), soil-pot, and field culture systems at 3 growth stages (flower, root swelling, and mature). The highest bioactive compound concentrations (danshensu (DSS), 0.618 mg·g^-1; salvianolic acid B (SAB), 52.5 mg·g^-1; cryptotanshinone (CTS), 0.617 mg·g^-1; tanshinoneⅡA (TSⅡA), 1.11 mg·g^-1; and total tanshinone (TTS), 2.5 mg·g^-1, at the mature stage) were present in the roots of plants grown in the hydroponic culture system. These concentrations were significantly higher than those of plants grown in the field system. The highest values for root parameters ( longest root length (LRL), 46.72 cm; largest root diameter (LRD), 14.68 mm; and the number of roots per plant (RN), 9.56), plant biomass (shoot dry weight (SDW), 18.9 g·plant^-1; root dry weight (RDW), 19.6 g·plant^-1, at the mature stage), and yield (DSS, 8.36 mg·plant^-1; SAB, 657 mg·plant^-1; CTS, 7.95 mg·plant^-1; TSⅡA, 15.2 mg·plant^-1; and TTS, 30.7 mg·plant^-1, at the mature stage) were obtained from plants grown in the field system. Plants grown in the field culture system had significantly greater plant biomass and higher yields of bioactive compounds than plants grown in the quartz sand-pot (hydroponic culture) and soil-pot systems. Greenhouse hydroponic culture provides sufficient bioactive compound accumulation in the roots, but does not stimulate plant growth and root production. Therefore, the field system could greatly improve plant growth and root production in S. miltiorrhiza.

Published in	Journal of Diseases and Medicinal Plants (Volume 4, Issue 3)
DOI	10.11648/j.jdmp.20180403.11
Page(s)	59-68
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Salvia miltiorrhiza, Culture System, Growth Stages, Bioactive Components, Growth

References

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Lilan Lu, Shilong Fu, Jian Feng, Jianhe Wei. (2018). Growth and Accumulation of Five Main Bioactive Components in the Roots of Salvia miltiorrhiza at Different Growth Stages and Using Different Culture Systems. Journal of Diseases and Medicinal Plants, 4(3), 59-68. https://doi.org/10.11648/j.jdmp.20180403.11

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ACS Style

Lilan Lu; Shilong Fu; Jian Feng; Jianhe Wei. Growth and Accumulation of Five Main Bioactive Components in the Roots of Salvia miltiorrhiza at Different Growth Stages and Using Different Culture Systems. J. Dis. Med. Plants 2018, 4(3), 59-68. doi: 10.11648/j.jdmp.20180403.11

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AMA Style

Lilan Lu, Shilong Fu, Jian Feng, Jianhe Wei. Growth and Accumulation of Five Main Bioactive Components in the Roots of Salvia miltiorrhiza at Different Growth Stages and Using Different Culture Systems. J Dis Med Plants. 2018;4(3):59-68. doi: 10.11648/j.jdmp.20180403.11

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@article{10.11648/j.jdmp.20180403.11,
  author = {Lilan Lu and Shilong Fu and Jian Feng and Jianhe Wei},
  title = {Growth and Accumulation of Five Main Bioactive Components in the Roots of Salvia miltiorrhiza at Different Growth Stages and Using Different Culture Systems},
  journal = {Journal of Diseases and Medicinal Plants},
  volume = {4},
  number = {3},
  pages = {59-68},
  doi = {10.11648/j.jdmp.20180403.11},
  url = {https://doi.org/10.11648/j.jdmp.20180403.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jdmp.20180403.11},
  abstract = {To Growth and accumulation of five main bioactive components in the roots of Salvia miltiorrhiza at different growth stages and using different culture systems. We analyzed growth parameters and the accumulation of selected bioactive components in Salvia miltiorrhiza that was grown in quartz sand-pot (hydroponic culture), soil-pot, and field culture systems at 3 growth stages (flower, root swelling, and mature). The highest bioactive compound concentrations (danshensu (DSS), 0.618 mg·g-1; salvianolic acid B (SAB), 52.5 mg·g-1; cryptotanshinone (CTS), 0.617 mg·g-1; tanshinoneⅡA (TSⅡA), 1.11 mg·g-1; and total tanshinone (TTS), 2.5 mg·g-1, at the mature stage) were present in the roots of plants grown in the hydroponic culture system. These concentrations were significantly higher than those of plants grown in the field system. The highest values for root parameters ( longest root length (LRL), 46.72 cm; largest root diameter (LRD), 14.68 mm; and the number of roots per plant (RN), 9.56), plant biomass (shoot dry weight (SDW), 18.9 g·plant-1; root dry weight (RDW), 19.6 g·plant-1, at the mature stage), and yield (DSS, 8.36 mg·plant-1; SAB, 657 mg·plant-1; CTS, 7.95 mg·plant-1; TSⅡA, 15.2 mg·plant-1; and TTS, 30.7 mg·plant-1, at the mature stage) were obtained from plants grown in the field system. Plants grown in the field culture system had significantly greater plant biomass and higher yields of bioactive compounds than plants grown in the quartz sand-pot (hydroponic culture) and soil-pot systems. Greenhouse hydroponic culture provides sufficient bioactive compound accumulation in the roots, but does not stimulate plant growth and root production. Therefore, the field system could greatly improve plant growth and root production in S. miltiorrhiza.},
 year = {2018}
}

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TY  - JOUR
T1  - Growth and Accumulation of Five Main Bioactive Components in the Roots of Salvia miltiorrhiza at Different Growth Stages and Using Different Culture Systems
AU  - Lilan Lu
AU  - Shilong Fu
AU  - Jian Feng
AU  - Jianhe Wei
Y1  - 2018/06/08
PY  - 2018
N1  - https://doi.org/10.11648/j.jdmp.20180403.11
DO  - 10.11648/j.jdmp.20180403.11
T2  - Journal of Diseases and Medicinal Plants
JF  - Journal of Diseases and Medicinal Plants
JO  - Journal of Diseases and Medicinal Plants
SP  - 59
EP  - 68
PB  - Science Publishing Group
SN  - 2469-8210
UR  - https://doi.org/10.11648/j.jdmp.20180403.11
AB  - To Growth and accumulation of five main bioactive components in the roots of Salvia miltiorrhiza at different growth stages and using different culture systems. We analyzed growth parameters and the accumulation of selected bioactive components in Salvia miltiorrhiza that was grown in quartz sand-pot (hydroponic culture), soil-pot, and field culture systems at 3 growth stages (flower, root swelling, and mature). The highest bioactive compound concentrations (danshensu (DSS), 0.618 mg·g-1; salvianolic acid B (SAB), 52.5 mg·g-1; cryptotanshinone (CTS), 0.617 mg·g-1; tanshinoneⅡA (TSⅡA), 1.11 mg·g-1; and total tanshinone (TTS), 2.5 mg·g-1, at the mature stage) were present in the roots of plants grown in the hydroponic culture system. These concentrations were significantly higher than those of plants grown in the field system. The highest values for root parameters ( longest root length (LRL), 46.72 cm; largest root diameter (LRD), 14.68 mm; and the number of roots per plant (RN), 9.56), plant biomass (shoot dry weight (SDW), 18.9 g·plant-1; root dry weight (RDW), 19.6 g·plant-1, at the mature stage), and yield (DSS, 8.36 mg·plant-1; SAB, 657 mg·plant-1; CTS, 7.95 mg·plant-1; TSⅡA, 15.2 mg·plant-1; and TTS, 30.7 mg·plant-1, at the mature stage) were obtained from plants grown in the field system. Plants grown in the field culture system had significantly greater plant biomass and higher yields of bioactive compounds than plants grown in the quartz sand-pot (hydroponic culture) and soil-pot systems. Greenhouse hydroponic culture provides sufficient bioactive compound accumulation in the roots, but does not stimulate plant growth and root production. Therefore, the field system could greatly improve plant growth and root production in S. miltiorrhiza.
VL  - 4
IS  - 3
ER  -

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Author Information

Lilan Lu

Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine, Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Haikou, China
Shilong Fu

Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine, Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Haikou, China
Jian Feng

Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine, Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Haikou, China
Jianhe Wei

Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine, Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Haikou, China

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APA Style

Lilan Lu, Shilong Fu, Jian Feng, Jianhe Wei. (2018). Growth and Accumulation of Five Main Bioactive Components in the Roots of Salvia miltiorrhiza at Different Growth Stages and Using Different Culture Systems. Journal of Diseases and Medicinal Plants, 4(3), 59-68. https://doi.org/10.11648/j.jdmp.20180403.11

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ACS Style

Lilan Lu; Shilong Fu; Jian Feng; Jianhe Wei. Growth and Accumulation of Five Main Bioactive Components in the Roots of Salvia miltiorrhiza at Different Growth Stages and Using Different Culture Systems. J. Dis. Med. Plants 2018, 4(3), 59-68. doi: 10.11648/j.jdmp.20180403.11

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AMA Style

Lilan Lu, Shilong Fu, Jian Feng, Jianhe Wei. Growth and Accumulation of Five Main Bioactive Components in the Roots of Salvia miltiorrhiza at Different Growth Stages and Using Different Culture Systems. J Dis Med Plants. 2018;4(3):59-68. doi: 10.11648/j.jdmp.20180403.11

Copy | Download

@article{10.11648/j.jdmp.20180403.11,
  author = {Lilan Lu and Shilong Fu and Jian Feng and Jianhe Wei},
  title = {Growth and Accumulation of Five Main Bioactive Components in the Roots of Salvia miltiorrhiza at Different Growth Stages and Using Different Culture Systems},
  journal = {Journal of Diseases and Medicinal Plants},
  volume = {4},
  number = {3},
  pages = {59-68},
  doi = {10.11648/j.jdmp.20180403.11},
  url = {https://doi.org/10.11648/j.jdmp.20180403.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jdmp.20180403.11},
  abstract = {To Growth and accumulation of five main bioactive components in the roots of Salvia miltiorrhiza at different growth stages and using different culture systems. We analyzed growth parameters and the accumulation of selected bioactive components in Salvia miltiorrhiza that was grown in quartz sand-pot (hydroponic culture), soil-pot, and field culture systems at 3 growth stages (flower, root swelling, and mature). The highest bioactive compound concentrations (danshensu (DSS), 0.618 mg·g-1; salvianolic acid B (SAB), 52.5 mg·g-1; cryptotanshinone (CTS), 0.617 mg·g-1; tanshinoneⅡA (TSⅡA), 1.11 mg·g-1; and total tanshinone (TTS), 2.5 mg·g-1, at the mature stage) were present in the roots of plants grown in the hydroponic culture system. These concentrations were significantly higher than those of plants grown in the field system. The highest values for root parameters ( longest root length (LRL), 46.72 cm; largest root diameter (LRD), 14.68 mm; and the number of roots per plant (RN), 9.56), plant biomass (shoot dry weight (SDW), 18.9 g·plant-1; root dry weight (RDW), 19.6 g·plant-1, at the mature stage), and yield (DSS, 8.36 mg·plant-1; SAB, 657 mg·plant-1; CTS, 7.95 mg·plant-1; TSⅡA, 15.2 mg·plant-1; and TTS, 30.7 mg·plant-1, at the mature stage) were obtained from plants grown in the field system. Plants grown in the field culture system had significantly greater plant biomass and higher yields of bioactive compounds than plants grown in the quartz sand-pot (hydroponic culture) and soil-pot systems. Greenhouse hydroponic culture provides sufficient bioactive compound accumulation in the roots, but does not stimulate plant growth and root production. Therefore, the field system could greatly improve plant growth and root production in S. miltiorrhiza.},
 year = {2018}
}

Copy | Download

TY  - JOUR
T1  - Growth and Accumulation of Five Main Bioactive Components in the Roots of Salvia miltiorrhiza at Different Growth Stages and Using Different Culture Systems
AU  - Lilan Lu
AU  - Shilong Fu
AU  - Jian Feng
AU  - Jianhe Wei
Y1  - 2018/06/08
PY  - 2018
N1  - https://doi.org/10.11648/j.jdmp.20180403.11
DO  - 10.11648/j.jdmp.20180403.11
T2  - Journal of Diseases and Medicinal Plants
JF  - Journal of Diseases and Medicinal Plants
JO  - Journal of Diseases and Medicinal Plants
SP  - 59
EP  - 68
PB  - Science Publishing Group
SN  - 2469-8210
UR  - https://doi.org/10.11648/j.jdmp.20180403.11
AB  - To Growth and accumulation of five main bioactive components in the roots of Salvia miltiorrhiza at different growth stages and using different culture systems. We analyzed growth parameters and the accumulation of selected bioactive components in Salvia miltiorrhiza that was grown in quartz sand-pot (hydroponic culture), soil-pot, and field culture systems at 3 growth stages (flower, root swelling, and mature). The highest bioactive compound concentrations (danshensu (DSS), 0.618 mg·g-1; salvianolic acid B (SAB), 52.5 mg·g-1; cryptotanshinone (CTS), 0.617 mg·g-1; tanshinoneⅡA (TSⅡA), 1.11 mg·g-1; and total tanshinone (TTS), 2.5 mg·g-1, at the mature stage) were present in the roots of plants grown in the hydroponic culture system. These concentrations were significantly higher than those of plants grown in the field system. The highest values for root parameters ( longest root length (LRL), 46.72 cm; largest root diameter (LRD), 14.68 mm; and the number of roots per plant (RN), 9.56), plant biomass (shoot dry weight (SDW), 18.9 g·plant-1; root dry weight (RDW), 19.6 g·plant-1, at the mature stage), and yield (DSS, 8.36 mg·plant-1; SAB, 657 mg·plant-1; CTS, 7.95 mg·plant-1; TSⅡA, 15.2 mg·plant-1; and TTS, 30.7 mg·plant-1, at the mature stage) were obtained from plants grown in the field system. Plants grown in the field culture system had significantly greater plant biomass and higher yields of bioactive compounds than plants grown in the quartz sand-pot (hydroponic culture) and soil-pot systems. Greenhouse hydroponic culture provides sufficient bioactive compound accumulation in the roots, but does not stimulate plant growth and root production. Therefore, the field system could greatly improve plant growth and root production in S. miltiorrhiza.
VL  - 4
IS  - 3
ER  -

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