Biofield Energy Based Vitamin D<sub>3</sub> Stimulates <i>In vitro</i> Osteoblasts Differentiation in MG-63 Cell line

Patricia Margaret Rowe; Mahendra Kumar Trivedi; Alice Branton; Dahryn Trivedi; Gopal Nayak; Mayank Gangwar; Snehasis Jana

doi:doi:10.11648/j.jdmp.20180401.12

Biofield Energy Based Vitamin D₃ Stimulates In vitro Osteoblasts Differentiation in MG-63 Cell line

Patricia Margaret Rowe, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis Jana

Published in Journal of Diseases and Medicinal Plants (Volume 4, Issue 1)

Received: 15 January 2018 Accepted: 1 February 2018 Published: 8 March 2018

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Abstract

In most of the people, bone loss and deterioration of bone structure results in bone disorders such as osteoporosis, arthritis, decreased bone mass, rickets, and deformed bones. Insufficient level of vitamin D₃ and calcium in the body are the major causes of bone disorders. The present study aimed to explore the potential of Consciousness Energy Healing based vitamin D₃ and DMEM medium on various bone health parameters such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization. Both the test items (TI) i.e. vitamin D₃ and DMEM medium were divided into two parts. The test items received the Consciousness Energy Healing Treatment by Patricia Margaret Rowe and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of both the sample were denoted as the untreated test items (UT). Cell viability using MTT assay showed that cell viability in tested samples was more than 70% with safe and nontoxic profile on MG-63 cell line. The level of ALP was significantly increased by 230.8% (at 50 µg/mL), 231.3% (at 50 µg/mL), and 253% (at 100 µg/mL) in UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively as compared with the untreated test item and DMEM group. Collagen level was significantly increased by 497.7%, 346.2%, 323%, and 331.4% at 0.1, 1, 10, and 50 µg/mL, respectively in the UT-DMEM+BT-TI group, while 116.3% and 112.8% at 0.1 and 50 µg/mL, respectively in the BT-DMEM+UT-TI group. In addition, 70.5% and 143.6% increased collagen was reported at 10 and 50 µg/mL, respectively in BT-DMEM+BT-TI group as compared with the untreated group. Bone mineralization percentage was significantly increased by 241.3% at 1 µg/mL in UT-DMEM+BT-TI group, while 187.9% and 113.7% at 0.1 and 1 µg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 129.8% at 1 µg/mL as compared with the untreated group. Overall, the experimental data suggested that the Biofield Energy Treated vitamin D₃ and DMEM would play an important role in the promotion and maintenance of strong and healthy bones, which improve quality of life. Biofield Energy Treatment might also regulates the osteoblast function, improves bone mineralization, and calcium absorption in wide range of bone disorders along with wide range of adverse health conditions, comprising cancer and certain autoimmune diseases.

Published in	Journal of Diseases and Medicinal Plants (Volume 4, Issue 1)
DOI	10.11648/j.jdmp.20180401.12
Page(s)	9-17
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Biofield Energy, Osteosarcoma Cells, Vitamin D₃, Osteoporosis, Bone Disorders, Bone Mineralization

References

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APA Style

Patricia Margaret Rowe, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, et al. (2018). Biofield Energy Based Vitamin D3 Stimulates In vitro Osteoblasts Differentiation in MG-63 Cell line. Journal of Diseases and Medicinal Plants, 4(1), 9-17. https://doi.org/10.11648/j.jdmp.20180401.12

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ACS Style

Patricia Margaret Rowe; Mahendra Kumar Trivedi; Alice Branton; Dahryn Trivedi; Gopal Nayak, et al. Biofield Energy Based Vitamin D3 Stimulates In vitro Osteoblasts Differentiation in MG-63 Cell line. J. Dis. Med. Plants 2018, 4(1), 9-17. doi: 10.11648/j.jdmp.20180401.12

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AMA Style

Patricia Margaret Rowe, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, et al. Biofield Energy Based Vitamin D3 Stimulates In vitro Osteoblasts Differentiation in MG-63 Cell line. J Dis Med Plants. 2018;4(1):9-17. doi: 10.11648/j.jdmp.20180401.12

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@article{10.11648/j.jdmp.20180401.12,
  author = {Patricia Margaret Rowe and Mahendra Kumar Trivedi and Alice Branton and Dahryn Trivedi and Gopal Nayak and Mayank Gangwar and Snehasis Jana},
  title = {Biofield Energy Based Vitamin D3 Stimulates In vitro Osteoblasts Differentiation in MG-63 Cell line},
  journal = {Journal of Diseases and Medicinal Plants},
  volume = {4},
  number = {1},
  pages = {9-17},
  doi = {10.11648/j.jdmp.20180401.12},
  url = {https://doi.org/10.11648/j.jdmp.20180401.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jdmp.20180401.12},
  abstract = {In most of the people, bone loss and deterioration of bone structure results in bone disorders such as osteoporosis, arthritis, decreased bone mass, rickets, and deformed bones. Insufficient level of vitamin D3 and calcium in the body are the major causes of bone disorders. The present study aimed to explore the potential of Consciousness Energy Healing based vitamin D3 and DMEM medium on various bone health parameters such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization. Both the test items (TI) i.e. vitamin D3 and DMEM medium were divided into two parts. The test items received the Consciousness Energy Healing Treatment by Patricia Margaret Rowe and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of both the sample were denoted as the untreated test items (UT). Cell viability using MTT assay showed that cell viability in tested samples was more than 70% with safe and nontoxic profile on MG-63 cell line. The level of ALP was significantly increased by 230.8% (at 50 µg/mL), 231.3% (at 50 µg/mL), and 253% (at 100 µg/mL) in UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively as compared with the untreated test item and DMEM group. Collagen level was significantly increased by 497.7%, 346.2%, 323%, and 331.4% at 0.1, 1, 10, and 50 µg/mL, respectively in the UT-DMEM+BT-TI group, while 116.3% and 112.8% at 0.1 and 50 µg/mL, respectively in the BT-DMEM+UT-TI group. In addition, 70.5% and 143.6% increased collagen was reported at 10 and 50 µg/mL, respectively in BT-DMEM+BT-TI group as compared with the untreated group. Bone mineralization percentage was significantly increased by 241.3% at 1 µg/mL in UT-DMEM+BT-TI group, while 187.9% and 113.7% at 0.1 and 1 µg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 129.8% at 1 µg/mL as compared with the untreated group. Overall, the experimental data suggested that the Biofield Energy Treated vitamin D3 and DMEM would play an important role in the promotion and maintenance of strong and healthy bones, which improve quality of life. Biofield Energy Treatment might also regulates the osteoblast function, improves bone mineralization, and calcium absorption in wide range of bone disorders along with wide range of adverse health conditions, comprising cancer and certain autoimmune diseases.},
 year = {2018}
}

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TY  - JOUR
T1  - Biofield Energy Based Vitamin D3 Stimulates In vitro Osteoblasts Differentiation in MG-63 Cell line
AU  - Patricia Margaret Rowe
AU  - Mahendra Kumar Trivedi
AU  - Alice Branton
AU  - Dahryn Trivedi
AU  - Gopal Nayak
AU  - Mayank Gangwar
AU  - Snehasis Jana
Y1  - 2018/03/08
PY  - 2018
N1  - https://doi.org/10.11648/j.jdmp.20180401.12
DO  - 10.11648/j.jdmp.20180401.12
T2  - Journal of Diseases and Medicinal Plants
JF  - Journal of Diseases and Medicinal Plants
JO  - Journal of Diseases and Medicinal Plants
SP  - 9
EP  - 17
PB  - Science Publishing Group
SN  - 2469-8210
UR  - https://doi.org/10.11648/j.jdmp.20180401.12
AB  - In most of the people, bone loss and deterioration of bone structure results in bone disorders such as osteoporosis, arthritis, decreased bone mass, rickets, and deformed bones. Insufficient level of vitamin D3 and calcium in the body are the major causes of bone disorders. The present study aimed to explore the potential of Consciousness Energy Healing based vitamin D3 and DMEM medium on various bone health parameters such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization. Both the test items (TI) i.e. vitamin D3 and DMEM medium were divided into two parts. The test items received the Consciousness Energy Healing Treatment by Patricia Margaret Rowe and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of both the sample were denoted as the untreated test items (UT). Cell viability using MTT assay showed that cell viability in tested samples was more than 70% with safe and nontoxic profile on MG-63 cell line. The level of ALP was significantly increased by 230.8% (at 50 µg/mL), 231.3% (at 50 µg/mL), and 253% (at 100 µg/mL) in UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively as compared with the untreated test item and DMEM group. Collagen level was significantly increased by 497.7%, 346.2%, 323%, and 331.4% at 0.1, 1, 10, and 50 µg/mL, respectively in the UT-DMEM+BT-TI group, while 116.3% and 112.8% at 0.1 and 50 µg/mL, respectively in the BT-DMEM+UT-TI group. In addition, 70.5% and 143.6% increased collagen was reported at 10 and 50 µg/mL, respectively in BT-DMEM+BT-TI group as compared with the untreated group. Bone mineralization percentage was significantly increased by 241.3% at 1 µg/mL in UT-DMEM+BT-TI group, while 187.9% and 113.7% at 0.1 and 1 µg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 129.8% at 1 µg/mL as compared with the untreated group. Overall, the experimental data suggested that the Biofield Energy Treated vitamin D3 and DMEM would play an important role in the promotion and maintenance of strong and healthy bones, which improve quality of life. Biofield Energy Treatment might also regulates the osteoblast function, improves bone mineralization, and calcium absorption in wide range of bone disorders along with wide range of adverse health conditions, comprising cancer and certain autoimmune diseases.
VL  - 4
IS  - 1
ER  -

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Author Information

Patricia Margaret Rowe

Trivedi Global, Inc., Henderson, USA
Mahendra Kumar Trivedi

Trivedi Global, Inc., Henderson, USA
Alice Branton

Trivedi Global, Inc., Henderson, USA
Dahryn Trivedi

Trivedi Global, Inc., Henderson, USA
Gopal Nayak

Trivedi Global, Inc., Henderson, USA
Mayank Gangwar

Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, India
Snehasis Jana

Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, India

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APA Style

Patricia Margaret Rowe, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, et al. (2018). Biofield Energy Based Vitamin D3 Stimulates In vitro Osteoblasts Differentiation in MG-63 Cell line. Journal of Diseases and Medicinal Plants, 4(1), 9-17. https://doi.org/10.11648/j.jdmp.20180401.12

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ACS Style

Patricia Margaret Rowe; Mahendra Kumar Trivedi; Alice Branton; Dahryn Trivedi; Gopal Nayak, et al. Biofield Energy Based Vitamin D3 Stimulates In vitro Osteoblasts Differentiation in MG-63 Cell line. J. Dis. Med. Plants 2018, 4(1), 9-17. doi: 10.11648/j.jdmp.20180401.12

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AMA Style

Patricia Margaret Rowe, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, et al. Biofield Energy Based Vitamin D3 Stimulates In vitro Osteoblasts Differentiation in MG-63 Cell line. J Dis Med Plants. 2018;4(1):9-17. doi: 10.11648/j.jdmp.20180401.12

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@article{10.11648/j.jdmp.20180401.12,
  author = {Patricia Margaret Rowe and Mahendra Kumar Trivedi and Alice Branton and Dahryn Trivedi and Gopal Nayak and Mayank Gangwar and Snehasis Jana},
  title = {Biofield Energy Based Vitamin D3 Stimulates In vitro Osteoblasts Differentiation in MG-63 Cell line},
  journal = {Journal of Diseases and Medicinal Plants},
  volume = {4},
  number = {1},
  pages = {9-17},
  doi = {10.11648/j.jdmp.20180401.12},
  url = {https://doi.org/10.11648/j.jdmp.20180401.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jdmp.20180401.12},
  abstract = {In most of the people, bone loss and deterioration of bone structure results in bone disorders such as osteoporosis, arthritis, decreased bone mass, rickets, and deformed bones. Insufficient level of vitamin D3 and calcium in the body are the major causes of bone disorders. The present study aimed to explore the potential of Consciousness Energy Healing based vitamin D3 and DMEM medium on various bone health parameters such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization. Both the test items (TI) i.e. vitamin D3 and DMEM medium were divided into two parts. The test items received the Consciousness Energy Healing Treatment by Patricia Margaret Rowe and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of both the sample were denoted as the untreated test items (UT). Cell viability using MTT assay showed that cell viability in tested samples was more than 70% with safe and nontoxic profile on MG-63 cell line. The level of ALP was significantly increased by 230.8% (at 50 µg/mL), 231.3% (at 50 µg/mL), and 253% (at 100 µg/mL) in UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively as compared with the untreated test item and DMEM group. Collagen level was significantly increased by 497.7%, 346.2%, 323%, and 331.4% at 0.1, 1, 10, and 50 µg/mL, respectively in the UT-DMEM+BT-TI group, while 116.3% and 112.8% at 0.1 and 50 µg/mL, respectively in the BT-DMEM+UT-TI group. In addition, 70.5% and 143.6% increased collagen was reported at 10 and 50 µg/mL, respectively in BT-DMEM+BT-TI group as compared with the untreated group. Bone mineralization percentage was significantly increased by 241.3% at 1 µg/mL in UT-DMEM+BT-TI group, while 187.9% and 113.7% at 0.1 and 1 µg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 129.8% at 1 µg/mL as compared with the untreated group. Overall, the experimental data suggested that the Biofield Energy Treated vitamin D3 and DMEM would play an important role in the promotion and maintenance of strong and healthy bones, which improve quality of life. Biofield Energy Treatment might also regulates the osteoblast function, improves bone mineralization, and calcium absorption in wide range of bone disorders along with wide range of adverse health conditions, comprising cancer and certain autoimmune diseases.},
 year = {2018}
}

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TY  - JOUR
T1  - Biofield Energy Based Vitamin D3 Stimulates In vitro Osteoblasts Differentiation in MG-63 Cell line
AU  - Patricia Margaret Rowe
AU  - Mahendra Kumar Trivedi
AU  - Alice Branton
AU  - Dahryn Trivedi
AU  - Gopal Nayak
AU  - Mayank Gangwar
AU  - Snehasis Jana
Y1  - 2018/03/08
PY  - 2018
N1  - https://doi.org/10.11648/j.jdmp.20180401.12
DO  - 10.11648/j.jdmp.20180401.12
T2  - Journal of Diseases and Medicinal Plants
JF  - Journal of Diseases and Medicinal Plants
JO  - Journal of Diseases and Medicinal Plants
SP  - 9
EP  - 17
PB  - Science Publishing Group
SN  - 2469-8210
UR  - https://doi.org/10.11648/j.jdmp.20180401.12
AB  - In most of the people, bone loss and deterioration of bone structure results in bone disorders such as osteoporosis, arthritis, decreased bone mass, rickets, and deformed bones. Insufficient level of vitamin D3 and calcium in the body are the major causes of bone disorders. The present study aimed to explore the potential of Consciousness Energy Healing based vitamin D3 and DMEM medium on various bone health parameters such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization. Both the test items (TI) i.e. vitamin D3 and DMEM medium were divided into two parts. The test items received the Consciousness Energy Healing Treatment by Patricia Margaret Rowe and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of both the sample were denoted as the untreated test items (UT). Cell viability using MTT assay showed that cell viability in tested samples was more than 70% with safe and nontoxic profile on MG-63 cell line. The level of ALP was significantly increased by 230.8% (at 50 µg/mL), 231.3% (at 50 µg/mL), and 253% (at 100 µg/mL) in UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively as compared with the untreated test item and DMEM group. Collagen level was significantly increased by 497.7%, 346.2%, 323%, and 331.4% at 0.1, 1, 10, and 50 µg/mL, respectively in the UT-DMEM+BT-TI group, while 116.3% and 112.8% at 0.1 and 50 µg/mL, respectively in the BT-DMEM+UT-TI group. In addition, 70.5% and 143.6% increased collagen was reported at 10 and 50 µg/mL, respectively in BT-DMEM+BT-TI group as compared with the untreated group. Bone mineralization percentage was significantly increased by 241.3% at 1 µg/mL in UT-DMEM+BT-TI group, while 187.9% and 113.7% at 0.1 and 1 µg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 129.8% at 1 µg/mL as compared with the untreated group. Overall, the experimental data suggested that the Biofield Energy Treated vitamin D3 and DMEM would play an important role in the promotion and maintenance of strong and healthy bones, which improve quality of life. Biofield Energy Treatment might also regulates the osteoblast function, improves bone mineralization, and calcium absorption in wide range of bone disorders along with wide range of adverse health conditions, comprising cancer and certain autoimmune diseases.
VL  - 4
IS  - 1
ER  -

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